Expression protéique fait référence à la synthèse de protéines within cells using information contained in the DNA or gene.
Protéines are responsible for all the biochemical reactions that take place within the cell. Hence, it makes it necessary to study de protéines function in order to understand cellular processes.
This is currently studied based on using fluorescent protéines as tags. However, this does not provide real time analysis as it requires maturation of chromophore that takes time and this causes a delay in investigation of real-time expression, especially for protéines qui sont de nature transitoire ou éphémère.
Les chercheurs ont signalé une nouvelle technique sur le serveur de préimpression le 30 juillet 2020 qui peut surmonter cette limitation.
The new study describes the use a fluorescent biosensor that enables real-time detection of de protéines expression in vivo which has implications for understanding the spatiotemporal expression of protéines within the living organism. This sensor is based on a dim green fluorescent de protéines in which pre-existing fluorescence increases 11-fold in vivo following the specific and rapid binding of a de protéines tag and enables detection of de protéines expression within seconds in live cells.
Ce biocapteur will be useful to study biological processes in real-time where protéines are either transiently expressed and/or to understand the expression and interactions of protéines from a disease-causing organism such as bacteria or a virus with that of the host proteins.
Référence:
Eason MG., Pandelieva AT., Mayer MM., et al 2020. Biocapteur fluorescent codé génétiquement pour la détection rapide de l'expression des protéines. Préimpression : bioRxiv 2020.07.30.229633 ; EST CE QUE JE: https://doi.org/10.1101/2020.07.30.229633
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